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KMID : 0363620090300010051
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Journal of Korean Oriental Medicine
2009 Volume.30 No. 1 p.51 ~ p.63
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Effects of Sotosaja-hwan on the Generation of ROS, RNS, and on the Expression of NF--dependent Proteins in ob/ob Mouse
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Bang Yong-Suk
Jung Ji-Chun
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Abstract
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Objectives: Peroxynitrite (), superoxide anion radical ( and nitric oxide (NO) are cytotoxic because they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer’s disease, rheumatoid arthritis, cancer, diabetes, obesity and atherosclerosis. The aim of this study was to investigate the , NO, scavenging and NF- related anti-inflammatory activities of Sotosaja-hwan in ob/ob mice.
Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice) and control obese (ob/ob mice) groups have received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho--, anti-IKK-, anti-NF- (p50, p65), anti-COX-2, anti-iNOS, anti-YCAM-1 and anti-MMP-9 antibodies, respectively.
Results: Sotosaja-hwan inhibited the generation of , NO and in the lipopolysaccharide (LPS)-treated mouse kidney postmitochondrial fraction in vitro. The generation of , NO, and PGE2 were inhibited in the Sotosaja-hwan-administered ob/ob mice groups. The GSH/GSSG ratio was decreased in the ob/ob mice, whereas the ratio was improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the protein expression levels of phospho--, IKK-, NF- (p50, p65), COX-2, iNOS, YCAM-1 and MMP-9 genes.
Conclusions: These results suggest that Sotosaja-hwan is an effective , and NO scavenger and has NF-kB related anti-inflammatory activity in ob/ob mice. Therefore, Sotosaja-hwan might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.
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KEYWORD
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Sotosaja-hwan, peroxynitrite, nitric oxide, reactive oxygen species, IKK-α, IκB-α, NF-κB, COX-2, iNOS
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